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Osteopontinele si rolul lor in angiogeneza

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Osteopontinele si rolul lor in angiogeneza Empty Osteopontinele si rolul lor in angiogeneza




Osteopontin (OPN) has been found to play an important role in tumor angiogenesis in recent years. Our previous studies have shown that OPN is overexpressed in tumor-associated human endometrial endothelial cells (HEECs) isolated from tissue samples of patients with endometrial cancer," scientists in Jinan, People's Republic of China report.
"In the present study, we aimed to further determine the role of OPN in endometrial cancer-associated angiogenesis. We knock down OPN expression in HEECs and human endometrial cancer Ishikawa (ISK) cells using the small interference RNA method, and then evaluate the effects of OPN oil endometrial cancer-associated angiogenesis by in vivo mouse studies and in vitro assays. Our results revealed that proliferative activity of HEECs and ISK cells in vitro was not affected by transfection with the siOPN-RNA (P >0.05).
Inhibition of OPN expression in HEECs reduced the cell migration, with the percentage of repaired area of 36.32 +/- 2.88 vs. 8.54 +/- 1.13 (P=0.007). HEEC/siOPN and ISK/siOPN demonstrated 67.4% and 51.2% decreased invasiveness compared with controls, respectively (P <0.05). The number of branched points per well was obviously lower in HEEC/siOPN than that in HEEC/Control (32.46 +/- 17.10 vs. 53.15 +/- 15.44, P=0.021). Furthermore, ISK cells transfected with OPN siRN!
A formed smaller tumor in mice and led to a lower microvessel density, i.e., angiogenesis, in transplanted tumors of mice than scrambled siRNA controls (12.88 +/- 7.14 vs. 28.42 +/- 9.69 vessels per HPF, P=0.019)," wrote X.L. Du and colleagues.

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